This antibody recognizes the Human CD8 cell surface antigen, a 32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T-cells and weakly by NK cells. Clone 4B11 has been reported as being suitable for use in Western blotting. AM05530SU-N is recommended for this purpose.
Immunohistochemistry on Frozen Sections: 1/20-1/40. Immunohistochemistry on paraffin sections: 1/20-1/40. This product requires antigenretrieval using heat treatment prior to staining of paraffin sections.1 mM EDTA pH 8.0 isrecommended for this purpose. Positive Control Tissue: Human Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
溶解方式
Restore with 1 mL distilled water.
储存条件
4 °C/-20 °C
储存方法
Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one monthor (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2