ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
交叉反应
小鸡, 人, 小鼠
产品特性
ENO2, Enolase 2, (gamma, neuronal), 2-Phospho-D-Glycerate Hydrolase,Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified, Enolase, Enolase and Enolase. Enolase expression has been detected on most tissues, whereas Enolase is expressed predominantly in muscle tissue and Enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway.
纯化方法
Protein A/G Chromatography
免疫原
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the human NSE protein and mouse myeloma Ag8563 cells. Sequence common in human, mouse and chicken.
Antibody can be used for Western blotting (1-2 μg/mL) and immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-5 μg/mL). Optimal concentration should be evaluated by serial dilutions.
限制
仅限研究用
缓冲液
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles