Sphingosine 1-phosphate (S1P) is a highly bioactive lipid that has a myriad of biological effects both intracellularly as a second messenger and extracellularly by binding to the S1P(1-5)/G-protein-coupled receptors of the endothelial differentiation gene family. Intracellularly, at least two enzymes, sphingosine kinase (1 and 2) and S1P phosphatase, regulate the activity of S1P by governing the phosphorylation of S1P. Regulation of hS1Pase1 with the resultant changes in cellular and secreted S1P has important implications in cell proliferation, angiogenesis and apoptosis.
纯化方法
Ammonium Sulfate Precipitation
免疫原
Synthetic peptide derived from the sphingosine 1-phosphate phosphatase 1 protein.
Antibody can be used for Western blotting (1:200 dilution). Tissue lysate should be used at 10-50 μg/lane. Other applications not yet tested. Optimal concentration should be evaluated by serial dilutions.
限制
仅限研究用
状态
Liquid
缓冲液
Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles