Western Blotting (WB), Immunohistochemistry (IHC), Inhibition Assay (InhA)
交叉反应
人
产品特性
GPR68, Ovarian Cancer G Protein-Coupled Receptor 1,OGR1 (originally identified as GPR68) is a high-affinity receptor for sphingosylphosphorylcholine (SPC). SPC is a member of a class of bioactive lipids which regulate cellular functions. These functions include cell proliferation and growth inhibition, smooth muscle contration and wound healing. SPC also stimulated signalling pathways that induce tyrosine phosphorylation, activate MAP kinases and protein kinase C, modify ion channel activity and increase intracellular Ca2+ concentration. OGR1 is expressed in placenta, lung, liver, spleen, testis, small intestine and peripheral blood leukocytes. It is not found in thymus, ovary, colon, skeletal muscle, kidney or pancreas.
纯化方法
Protein A/G Chromatography
免疫原
Rabbits were immunized with recombinant protein derived from human OGR1 (GPR68).
Antibody can be used for Western blotting (5-10 μg/mL). Western blot should be visualized using a high sensitivity secondary/substrate system (i.e. Pierce's SuperSignal West Femto Maximum Sensitivity Substrate). Optimal concentration should be evaluated by serial dilutions.
限制
仅限研究用
缓冲液
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles