Serine/threonine-protein phosphatase 5 (EC 3.1.3.16) (PP5) (Protein phosphatase T) (PP-T) (PPT), PPP5C, PPP5,Protein phosphatase 5 (PP5) is a novel human protein serine/threonine phosphatase of molecular weight of 58 kDa. It is made up of a C-terminal phosphatase catalytic domain and an N-terminal domain, which has four repeats of 34 amino acids, three of which are tandemly arranged. The phosphatase domain contains all of the invariant motifs of the PP1/PP2A/PP2B gene family. However, it is not closely related to any other known member of this family of phosphatases. Thus it makes up a new subfamily of phosphatases. PP5 expressed in bacteria has been shown to dephosphorylate serine residues in proteins and is more sensitive than PP1 to the tumor promoter okadaic acid. PP5 appears to be localized to the nucleus suggesting that, like other nuclear tetratricopeptide (TPR)-containing proteins, it may playa role in the regulation of RNA biogenesis and mitosis.
纯化方法
Ammonium Sulfate Precipitation
免疫原
Rabbits were immunized with a Synthetic peptide derived from the human protein phosphatase 5 proteins
This antibody can be used for Western blotting (5-10 μg/mL). Other applications being evaulated. Optimal concentration should be evaluated by serial dilutions.
限制
仅限研究用
缓冲液
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles