Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Gel Shift (GS)
交叉反应
人, 小鼠, 大鼠
产品特性
Myogenin, Myogenic factor 4, Myf-4,Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.
纯化方法
Protein A/G Chromatography
免疫原
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.
This antibody can be used for ELISA, gel supershift assays, immunofluorescence, immunoprecipitation of protein-DNA complexes (2 μg/mg), Western blotting (1 μg/mL) and is the antibody of choise for immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-2 μg/mL). Optimal concentration should be evaluated by serial dilutions.
限制
仅限研究用
缓冲液
Provided as solution in phosphate buffered saline with 0.08 % sodium azide,X1415M, clone F12B recognizes a protein of 34 kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles