Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
特异性
Human Chx10 protein
交叉反应
小鸡, 人, 小鼠, 大鼠
产品特性
Ceh-10 homeodomain-containing homolog,Chx10 is a 46 kDa homeodomain protein of the paired-like class that is essential for development of the mammalian eye. Mutations in Chx10 cause microphthalmia, a cause of congenital blindness in humans, and the ocular retardation (or) phenotype in mice. In the developing mouse retina Chx10 is expressed in retinal progenitors, while in the mature retina, Chx10 expression becomes restricted to bipolar neurons. Concurrent with these expression patterns, the Chx10-/- (or) retina is thin due to a defect in proliferation of retinal progenitors, and lacks bipolar neurons. Chx10 is also expressed in the developing brainstem, thalamus, and spinal cord.
免疫原
Antibody raised in sheep against a recombinant protein corresponding to amino acids 1 to 131 derived from the N-terminal region of the human Chx10 protein conjugated to KLH.
Detects Chx10 by Western blot at 0.5 to 1 μg/mL. Detects a 46 kDa band in mouse and rat retinal tissue lysates. Optimal concentration should be evaluated by serial dilutions. Suitable for use against recombinant proteins conjugated to OVA, GST, His tags and other.
限制
仅限研究用
缓冲液
Ammonium sulphate precipitated antibody in PBS with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles