Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
交叉反应
小鼠
产品特性
Rho guanine nucleotide exchange factor 2, ARHGEF2,Lfc is a 118 kDa protein discovered in a screen for oncogenes in NIH 3T3 cells. Lfc is a signaling protein related to Lbc and is part of the Dbl family of proteins. Lfc is a guanine-nucleotide exchange factor (GEF) for the Rho family of small GTPases. Lfc has also been shown to be upstream of the serine-threonine kinase JNK. Lfc has also been shown to mediate the activation of the Rac signaling pathway.
纯化方法
Ammonium Sulfate Precipitation
免疫原
Antibody developed using protein corresponding to amino acids 2 to 447 of the Lfc protein fused to GST.
Detects Lfc in mouse tissues by immunoprecipitation-Western blot at 4 μg/mL. Detects a 118 kDa band in mouse tissue extracts corresponding to wild-type Lfc. Can also be used in immunofluorescence assays. Optimal concentration should be evaluated by serial dilutions.
限制
仅限研究用
缓冲液
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles