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NAPA 抗体

NAPA 适用: 人 WB, IP, EIA 宿主: 小鼠 Monoclonal 15D4 unconjugated
产品编号 ABIN306712
发货至: 中国
  • 抗原 See all NAPA 抗体
    NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
    适用
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    宿主
    • 44
    • 4
    小鼠
    克隆类型
    • 43
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    单克隆
    标记
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    This NAPA antibody is un-conjugated
    应用范围
    • 32
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    Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
    特异性
    Human alpha -SNAP
    交叉反应
    Cow, 人, 大鼠
    产品特性
    alpha/beta SNAP, mouse anti alpha soluble NSF attachment protein,SNAPA,SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. Beta-SNAP (or SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas alpha-SNAP is expressed in all mammalian tissues, beta-SNAP is expressed only in brain.
    纯化方法
    The antibody was purified to >95% homogeneity by standard chromatographic techniques
    免疫原
    Balb/C mice were immunized with recombinant human alpha -SNAP. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by Western blot.,Monoclonal antibody 15D4 obtained immunization of Balb/C mice with recombinant human alpha -SNAP
    克隆位点
    15D4
    亚型
    IgG1
    Top Product
    Discover our top product NAPA Primary Antibody
  • 应用备注
    Optimal concentration should be evaluated by serial dilutions. Clone 4E4 anti-?SNAP specifically recognizes ?-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and does not recognize ?-SNAP Application: A dilution of 0.5-5 μg/mL is suggested for western blot and 2-10 μg/mL for immunoprecipitation.
    限制
    仅限研究用
  • 缓冲液
    0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol. No preservatives have been included in the antibody solution.,100 μg monoclonal antibody in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol. No preservatives have been added.
    储存液
    Without preservative
    储存条件
    -20 °C
    储存方法
    Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
  • 抗原
    NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
    别名
    alpha SNAP (NAPA 产品)
    别名
    SNAPA antibody, wu:fa26c04 antibody, zgc:63906 antibody, napb antibody, 1500039N14Rik antibody, AW209189 antibody, RA81 antibody, SNARE antibody, a-SNAP antibody, hyh antibody, alpha-SNAP antibody, Alpha-SNAP antibody, NSF attachment protein alpha antibody, N-ethylmaleimide-sensitive factor attachment protein, alpha b antibody, NSF attachment protein alpha L homeolog antibody, N-ethylmaleimide-sensitive factor attachment protein, alpha antibody, N-ethylmaleimide sensitive fusion protein attachment protein alpha antibody, soluble NSF attachment protein antibody, NAPA antibody, napab antibody, napa.L antibody, napa antibody, EHI_000300 antibody, Napa antibody, LOC102577508 antibody
    途径
    Synaptic Vesicle Exocytosis, Asymmetric Protein Localization
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