Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
特异性
Human alpha -SNAP
交叉反应
Cow, 人, 大鼠
产品特性
alpha/beta SNAP, mouse anti alpha soluble NSF attachment protein,SNAPA,SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. Beta-SNAP (or SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas alpha-SNAP is expressed in all mammalian tissues, beta-SNAP is expressed only in brain.
纯化方法
The antibody was purified to >95% homogeneity by standard chromatographic techniques
免疫原
Balb/C mice were immunized with recombinant human alpha -SNAP. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by Western blot.,Monoclonal antibody 15D4 obtained immunization of Balb/C mice with recombinant human alpha -SNAP
NAPA
适用: 人
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 488
应用备注
Optimal concentration should be evaluated by serial dilutions. Clone 4E4 anti-?SNAP specifically recognizes ?-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and does not recognize ?-SNAP Application: A dilution of 0.5-5 μg/mL is suggested for western blot and 2-10 μg/mL for immunoprecipitation.
限制
仅限研究用
缓冲液
0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol. No preservatives have been included in the antibody solution.,100 μg monoclonal antibody in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol. No preservatives have been added.
储存液
Without preservative
储存条件
-20 °C
储存方法
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles