Phosphotyrosine 抗体 (FITC)
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- 抗原 See all Phosphotyrosine products
- Phosphotyrosine
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Phosphotyrosine antibody is conjugated to FITC
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- 产品特性
- Mouse anti phosphotyrosine, PY20, FITC conjugate,The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
- 纯化方法
- Prot A/G
- 免疫原
- Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.
- 克隆位点
- PY20
- 亚型
- IgG2b
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anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibodyWB, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 3F6 unconjugated
anti-Phosphotyrosine antibodyWB, IP, IF, IHC, FACS, ISt, StM 宿主: 小鼠 Monoclonal PY20 unconjugated
anti-Phosphotyrosine antibodyWB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 unconjugated
anti-Phosphotyrosine antibody (HRP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 HRP
anti-Phosphotyrosine antibody (FITC)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 FITC
anti-Phosphotyrosine antibody (PerCP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 PerCP
anti-Phosphotyrosine antibody (Atto 594)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Atto 594
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- 应用备注
- A dilution of 5-10 μg/mL is suggested for immunohistochemistry or immunofluorescence. Optimal concentration should be evaluated by serial dilutions.
- 限制
- 仅限研究用
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- 缓冲液
- Provided as sterile solution in 20 mM sodium phosphate, 150 mM sodium chloride, 50 % glycerol, 3 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- 抗原
- Phosphotyrosine
- Abstract
- Phosphotyrosine 产品
- 物质类
- Amino Acid
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