CAPN1 抗体
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- 抗原 See all CAPN1 抗体
- CAPN1 (Calpain 1 (CAPN1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CAPN1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- 交叉反应
- Cow, 人
- 产品特性
- Mouse anti calpain I, II small subunit, p30,The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and μ-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, μ-calpain is active at micromolar (μM) concentrations of Ca ++. In addition to the ubiquitously expressed m- and μcalpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
- 纯化方法
- Protein A/G Chromatography
- 免疫原
- Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain with mouse myeloma.
- 克隆位点
- 28F3
- 亚型
- IgG1
- Top Product
- Discover our top product CAPN1 Primary Antibody
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- 应用备注
- Use at 0.5-1 μg/mL for ELISA and western blot, 1-2 μg/mL for immunoprecipitation.
- 限制
- 仅限研究用
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- 缓冲液
- Provided as a concentration of 1 mg/mL in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50 % glycerol, 3 mM sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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