TJP2 抗体 (C-Term)
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- 抗原 See all TJP2 抗体
- TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))
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抗原表位
- C-Term
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适用
- 人, 小鼠, 大鼠, 犬
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TJP2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Recognizes the ~130-160kD human ZO-2 protein, isoforms A or C. A band of unknown origin lower than 75kD may be observed in certain lysates.
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide derived from the C-terminal region of human or canine ZO-2.
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product TJP2 Primary Antibody
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- 应用备注
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Approved: IF (2.5 μg/mL), IHC, IHC-P (5 - 10 μg/mL), WB (1 - 3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Immunofluorescence: 2.5 μg/mL. Cells need to be fixed with ice-cold ethanol for 30 minutes on ice prior to assays. Positive control: Western Blot: CaCO2 human colorectal adenocarcinoma, MDCK II canine kidney, KNRK rat kidney, and TMCK-1 mouse kidney cell lysates. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.4, 0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Aliquot to Avoid repeated freezing and thawing
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C, Long term: aliquot to Avoid freeze-thaw cycles, and store at -20°C.
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- 抗原
- TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))
- 别名
- TJP2 / ZO2 / ZO-2 (TJP2 产品)
- 别名
- C9DUPq21.11 antibody, DFNA51 antibody, DUP9q21.11 antibody, X104 antibody, ZO2 antibody, ZO-2 antibody, zo2 antibody, tjp2 antibody, x104 antibody, zo-2 antibody, wu:fb62b09 antibody, zgc:92094 antibody, tight junction protein 2 antibody, tight junction protein 2 L homeolog antibody, tight junction protein 2b (zona occludens 2) antibody, TJP2 antibody, Tjp2 antibody, tjp2.L antibody, tjp2b antibody
- 背景
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Name/Gene ID: TJP2
Family: Occludin
Synonyms: TJP2, C9DUPq21.11, DUP9q21.11, DFNA51, ZO-2, Zona occludens 2, Zonula occludens protein 2, Tight junction protein 2, Tight junction protein ZO-2, ZO2, X104, Zona occludens protein 2 - 基因ID
- 9414
- UniProt
- Q9UDY2
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