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CD45RB 抗体 (FITC)

CD45RB 适用: 小鼠 FACS, IF 宿主: 大鼠 Monoclonal C363-16A FITC
产品编号 ABIN3044640
发货至: 中国
  • 抗原 See all CD45RB 抗体
    CD45RB
    适用
    • 58
    • 23
    • 7
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    • 3
    • 3
    • 2
    小鼠
    宿主
    • 47
    • 23
    • 11
    大鼠
    克隆类型
    • 81
    单克隆
    标记
    • 22
    • 8
    • 8
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    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 1
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    This CD45RB antibody is conjugated to FITC
    应用范围
    • 78
    • 40
    • 39
    • 26
    • 18
    • 17
    • 6
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF)
    产品特性
    Monoclonal Anti-Mouse CD45RB FITC is recommended for use in flow cytometry for identification of CD45RB, an isoform of CD45. The anti-mouse CD45RB monoclonal antibody reacts with the CD45 isoform containing the exon B dependent epitope. CD45RB is highly expressed on peripheral B cells, cytotoxic T cells, a subset of T helper cells and most thymoctyes.
    免疫原
    Cloned mouse Th2 cell lines.
    克隆位点
    C363-16A
    亚型
    IgG2a
    Top Product
    Discover our top product CD45RB Primary Antibody
  • 应用备注
    It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using less than or equal to 0,25 µg/10^6 cells
    说明

    Fluorescein Isothiocyanate, FITC (Abs/Em: 495/ 517 nm).

    试剂准备
    1. Transfer the sample of blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add anti-mouse CD45RB FITC and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
    储存液
    Sodium azide
    注意事项
    1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
    储存条件
    4 °C
  • 抗原
    CD45RB
    别名
    CD45RB (CD45RB 产品)
    别名
    B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, PTPRC antibody, Ptprc antibody
    背景
    CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions.
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