TCR gamma/delta 抗体 (FITC)
(1 reference)-
- 抗原 See all TCR gamma/delta products
- TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))
- 适用
- 请咨询
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宿主
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Armenian Hamster
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克隆类型
- 单克隆
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标记
- This TCR gamma/delta antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 免疫原
- Proprietary Immunogen Species
- 克隆位点
- UC7-13D5
- 亚型
- IgG
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anti-T-Cell Receptor gamma/delta (TCR gamma/delta) antibody
TCR gamma/delta 适用: 大鼠 FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal V65 unconjugated
anti-T-Cell Receptor gamma/delta (TCR gamma/delta) antibody (FITC)TCR gamma/delta 适用: 人 FACS 宿主: 小鼠 Monoclonal 11F2 FITC
anti-T-Cell Receptor gamma/delta (TCR gamma/delta) antibody (PE)TCR gamma/delta 适用: 人 FACS 宿主: 小鼠 Monoclonal 11F2 PE
anti-T-Cell Receptor gamma/delta (TCR gamma/delta) antibody (APC)TCR gamma/delta 适用: 人 FACS 宿主: 小鼠 Monoclonal 11F2 APC
anti-T-Cell Receptor gamma/delta (TCR gamma/delta) antibody (PE-DyLight 594)TCR gamma/delta 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal B1 PE-DyLight 594
anti-T-Cell Receptor gamma/delta (TCR gamma/delta) antibody (PE)TCR gamma/delta 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal V65 PE
anti-T-Cell Receptor gamma/delta (TCR gamma/delta) antibodyTCR gamma/delta 适用: 大鼠 FACS, IP, IHC (fro), Func 宿主: 小鼠 Monoclonal V65 unconjugated
anti-T-Cell Receptor gamma/delta (TCR gamma/delta) antibodyTCR gamma/delta 适用: 人 FACS, IP, IHC (fro), ELISA 宿主: 小鼠 Monoclonal 11F2 unconjugated
anti-T-Cell Receptor gamma/delta (TCR gamma/delta) antibodyTCR gamma/delta 适用: 人, Non-Human Primate FACS, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal B1 unconjugated
anti-T-Cell Receptor gamma/delta (TCR gamma/delta) antibodyTCR gamma/delta 适用: 人, Non-Human Primate FACS, IHC (fro), Func, IHC (p) 宿主: 小鼠 Monoclonal B1 unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using 1 µg/10^6 cells
- 说明
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Fluorescein Isothiocyanate, FITC (Abs/Em: 495/ 517 nm).
- 试剂准备
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- Transfer the sample to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add anti-mouse anti-mouse TCR gamma/delta FITC and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, (1995) (PubMed).
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: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, (1995) (PubMed).
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- 抗原
- TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))
- 别名
- TCR gamma/delta (TCR gamma/delta 产品)
- 背景
- T cell receptor (TCR) is a heterodimer consisting of an alpha and a beta chain (TCR alpha/beta) or a gamma and a delta chain (TCR gama/delta). TCR-beta is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-alpha). It is expressed on alpha/beta TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.
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