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Nestin 抗体 (N-Term)

Name: Nestin 抗体 (N-Term)
SKU: ABIN3044529
Availability: OutOfStock

NES 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

Western blot analysis of Nestin expression in HELA whole cell lysates (lane 1) and HEPG2 whole cell lysates (lane 2). (Nestin 抗体 (N-Term))

Nestin was detected in paraffin-embedded sections of human melanoma tissues using rabbit anti- Nestin Antigen Affinity purified polyclonal antibody at 1 μg/mL. (Nestin 抗体 (N-Term))

IHC (p)

Nestin was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti- Nestin Antigen Affinity purified polyclonal antibody at 1 μg/mL. (Nestin 抗体 (N-Term))

IHC (p)

IHC analysis of Nestin using anti-Nestin antibody . (Nestin 抗体 (N-Term))

IHC

5 images

(18 references)

产品编号 ABIN3044529

发货至: 中国

Datasheet as PDF

抗原 See all Nestin (NES) 抗体

Nestin (NES)
抗原表位
16

16

15

10

8

7

6

5

5

4

3

3

2

2

2

2

1

1

1

1

1

1

1

1
AA 252-290, N-Term
适用
136

73

51

4

3

2

2

2

1

1

1

1
人, 大鼠
宿主
108

36

3

1

1
兔
Compatible Secondaries
克隆类型
113

36
多克隆
标记
61

10

9

7

7

4

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3
This Nestin antibody is un-conjugated
应用范围
73

48

48

48

41

39

35

26

25

16

9

6

2

2

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

原理

Rabbit IgG polyclonal antibody for Protein Mdm4(MDM4) detection. Tested with WB, IHC-P in Human,Rat.

序列

ERLRATEKFQ LAVEALEQEK QGLQSQIAQV LEGRQQLAH

交叉反应（详细）

No cross reactivity with other proteins.

产品特性

Rabbit IgG polyclonal antibody for Protein Mdm4(MDM4) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: nestin
Protein Name: Nestin

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human Nestin (252-290aa ERLRATEKFQLAVEALEQEKQGLQSQIAQVLEGRQQLAH), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

亚型

IgG

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anti-Nestin (NES) antibody (FITC)
NES 适用: 人 IF, ICC 宿主: 小鼠 Monoclonal 10C2 FITC

anti-Nestin (NES) (AA 1392-1621) antibody
NES 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Li, Xie, Yu, He: "Increased Efficacy of Antivenom Combined with Hyperbaric Oxygen on Deinagkistrodon acutus Envenomation in Adult Rats." in: Chinese medical journal, Vol. 131, Issue 3, pp. 323-329, (2018) (PubMed).

Luo, Deng, Yi, Zhou, She, Liu: "Buyang Huanwu Decoction Ameliorates Poststroke Depression via Promoting Neurotrophic Pathway Mediated Neuroprotection and Neurogenesis." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 4072658, (2017) (PubMed).

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Yang, Li, Li, Feng, Liu, Han, Zhang, Qin, Zhang, Nie, Jin: "Effect of Corilagin on the Proliferation and NF-κB in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 1418309, (2016) (PubMed).

Zeng, Zhao, Ouyang, Zhao, Zhang, Chen, Yu, Lei: "Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium." in: Oncology letters, Vol. 12, Issue 2, pp. 815-824, (2016) (PubMed).

Li, Lu, Wang: "Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells." in: Neural regeneration research, Vol. 9, Issue 22, pp. 2002-7, (2015) (PubMed).

Lu, Li, Lu: "Distribution and localization of fibroblast growth factor-8 in rat brain and nerve cells during neural stem/progenitor cell differentiation." in: Neural regeneration research, Vol. 7, Issue 19, pp. 1455-62, (2015) (PubMed).

Chen, Zhang, Liu, He, Zhou, Shao, Sun, Cao, Gong, Jiang: "A fibrin matrix promotes the differentiation of EMSCs isolated from nasal respiratory mucosa to myelinating phenotypical Schwann-like cells." in: Molecules and cells, Vol. 38, Issue 3, pp. 221-8, (2015) (PubMed).

Liu, Guo, Zhang, Li, Feng, Yu, Kong, Zhao, Jin: "Effect of siRNA-Livin on drug resistance to chemotherapy in glioma U251 cells and CD133+ stem cells." in: Experimental and therapeutic medicine, Vol. 10, Issue 4, pp. 1317-1323, (2015) (PubMed).

Wei, Yang, Hou, Liu, Gao, Zhang, Wang, Mao, Gao, Chen, Hong: "Insulin-producing cells derived from human embryonic stem cells: comparison of definitive endoderm- and nestin-positive progenitor-based differentiation strategies." in: PLoS ONE, Vol. 8, Issue 8, pp. e72513, (2014) (PubMed).

Xiong, Zhou, Wang, Xue, Wang, Sun, Ge: "Impaired spatial learning and memory after sevoflurane-nitrous oxide anesthesia in aged rats is associated with down-regulated cAMP/CREB signaling." in: PLoS ONE, Vol. 8, Issue 11, pp. e79408, (2014) (PubMed).

Zhang, Pei, Ru, Li, Liu: "Bone morphogenetic protein 7 upregulates the expression of nestin and glial fibrillary acidic protein in rats with cerebral ischemia-reperfusion injury." in: Biomedical reports, Vol. 1, Issue 6, pp. 895-900, (2014) (PubMed).

Xu, Sun, Wu, Xi: "Investigation of characteristics of feather follicle stem cells and their regeneration potential." in: Journal of stem cells & regenerative medicine, Vol. 7, Issue 2, pp. 69-74, (2014) (PubMed).

Fu, Lv, Zhuang, Chen, Lv, Li, Wang: "An effective inducer of dopaminergic neuron-like differentiation." in: Neural regeneration research, Vol. 8, Issue 5, pp. 427-34, (2014) (PubMed).

Yang, Zhu, Zhang, Gao, Cao: "Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats." in: Neural regeneration research, Vol. 7, Issue 34, pp. 2698-704, (2014) (PubMed).

Hu, Deng, Liu, Chen, Zhang, Sun, Dong, Liu, Ge: "Characterization and retinal neuron differentiation of WERI-Rb1 cancer stem cells." in: Molecular vision, Vol. 18, pp. 2388-97, (2013) (PubMed).

Bin, Ma, Xu, Shi: "Embryonic hepatocyte transplantation for hepatic cirrhosis: efficacy and mechanism of action." in: World journal of gastroenterology, Vol. 18, Issue 4, pp. 309-22, (2012) (PubMed).

Yang, Zhou, Wang, Wang: "Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigmented epithelial cells." in: International journal of ophthalmology, Vol. 3, Issue 1, pp. 23-7, (2012) (PubMed).
抗原

Nestin (NES)

别名

NES (NES 产品)

别名

paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody

背景

Nestin is a type VI intermediate filament (IF) protein that in humans is encoded by the NES gene. This gene is mapped to 1q23.1. Distribution and expression of nestin in mitotically active cells suggests it plays a role in regulation of the assembly and disassembly of intermediate filaments, which, together with other structural proteins, participate in remodeling of the cell. The role of nestin in dynamic cells, particularly structural organization of the cell, appears strictly regulated by phosphorylation, especially its integration into heterogeneous intermediate filaments together with vimentin or α-internexin. Furthermore, nestin expression has been extensively used as a marker for central nervous system (CNS) progenitor cells in different contexts, based on observations indicating a correlation between nestin expression and this cell type in vivo.

Synonyms: ESTM 46 antibody|FLJ 21841 antibody|FLJ21841 antibody|Intermediate filament protein antibody|Nbla00170 antibody|nes antibody| NEST_HUMAN antibody|Nestin antibody

基因ID

10763

UniProt

P48681