BRCA1 抗体 (AA 1661-1863)
(2 references)Quick Overview for BRCA1 抗体 (AA 1661-1863) (ABIN3044313)
抗原
See all BRCA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1661-1863
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原理
- Anti-BRCA1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-BRCA1 Antibody Picoband® (ABIN3044313). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human BRCA1 recombinant protein (Position: E1661-Y1863). Human BRCA1 shares 65% and 66% amino acid (aa) sequences identity with mouse and rat BRCA1, respectively.
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亚型
- IgG
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anti-Breast Cancer 1 (BRCA1) (AA 19-83) antibody
BRCA1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer 1 (BRCA1) (pSer1423) antibodyBRCA1 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer 1 (BRCA1) (AA 229-335) antibodyBRCA1 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 6C6D2 unconjugated
anti-Breast Cancer 1 (BRCA1) (Ser184), (Ser186) antibodyBRCA1 适用: 人, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer 1 (BRCA1) (AA 445-620) antibodyBRCA1 适用: 人 IHC, ISt, StM 宿主: 小鼠 Monoclonal BRCA1-1398 unconjugated
anti-Breast Cancer 1 (BRCA1) (AA 1314-1600), (AA 1314-1864) antibodyBRCA1 适用: 人 WB, IF, IP, FACS, ICC 宿主: 小鼠 Monoclonal RAY unconjugated
anti-Breast Cancer 1 (BRCA1) (AA 64-160) antibodyBRCA1 适用: 大鼠, 小鼠 ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer 1 (BRCA1) (pSer1524) antibodyBRCA1 适用: 人 ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer 1 (BRCA1) (AA 1524-1781) antibodyBRCA1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer 1 (BRCA1) (pSer1524) antibodyBRCA1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Lee, M.-H., Lahusen, T., Wang, R.-H., Xiao, C., Xu, X., Hwang, Y.-S., He, W.-W., Shi, Y., Deng, C.-X. Yin Yang 1 positively regulates BRCA1 and inhibits mammary cancer formation. Oncogene 31: 116-127, 2012. 2. Thompson, M. E., Jensen, R. A., Obermiller, P. S., Page, D. L., Holt, J. T. Decreased expression of BRCA1 accelerates growth and is often present during sporadic breast cancer progression. Nature Genet. 9: 444-450, 1995. 3. Wang, B., Hurov, K., Hofmann, K., Elledge, S. J. NBA1, a new player in the Brca1 A complex, is required for DNA damage resistance and checkpoint control. Genes Dev. 23: 729-739, 2009. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro." in: Cell death & disease, Vol. 8, Issue 8, pp. e2966, (2017) (PubMed).
: "The ectopic expression of BRCA1 is associated with genesis, progression, and prognosis of breast cancer in young patients." in: Diagnostic pathology, Vol. 7, pp. 181, (2013) (PubMed).
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: "Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro." in: Cell death & disease, Vol. 8, Issue 8, pp. e2966, (2017) (PubMed).
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- BRCA1 (Breast Cancer 1 (BRCA1))
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别名
- BRCA1
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背景
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Synonyms: Breast cancer type 1 susceptibility protein,6.3.2.-,RING finger protein 53,BRCA1,RNF53,
Tissue Specificity: Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.
Background: BRCA1, also known as BRCC1, is a gene which mapping to 17q21.3. This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). BRCA1 product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. In addition to it, BRCA1 may normally serve as a negative regulator of mammary epithelial cell growth and that this function is compromised in breast cancer either by mutation or by alterations in gene expression.
Sequence Similarities: Contains 2 BRCT domains.
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分子量
- 290 kDa
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基因ID
- 672
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UniProt
- P38398
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途径
- Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus
抗原
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