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Paxillin 抗体 (C-Term)

Name: Paxillin 抗体 (C-Term)
SKU: ABIN3044187
Availability: OutOfStock

PXN 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

Anti-Paxillin antibody, Western blotting Lane 1: 293T Cell Lysate Lane 2: HELA Cell Lysate Lane 3: MCF-7 Cell Lysate Lane 4: MM231 Cell Lysate Lane 5: JUKAT Cell Lysate (Paxillin 抗体 (C-Term))

Anti-Paxillin antibody, IHC(F) IHC(F): Rat Kidney Tissue (Paxillin 抗体 (C-Term))

IHC

Anti-Paxillin antibody, IHC(P) IHC(P): Human Lung Cancer Tissue (Paxillin 抗体 (C-Term))

IHC

Anti-Paxillin antibody, ICC ICC: HELA Cell (Paxillin 抗体 (C-Term))

IHC

4 images

(3 references)

产品编号 ABIN3044187

发货至: 中国

Datasheet as PDF

抗原 See all Paxillin (PXN) 抗体

Paxillin (PXN)
抗原表位
31

26

17

16

11

9

7

6

6

6

5

4

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 456-472, C-Term
适用
187

100

69

15

8

7

5

4

3

3

1

1

1

1

1
人, 小鼠, 大鼠
宿主
182

22

1
兔
Compatible Secondaries
克隆类型
172

33
多克隆
标记
108

10

6

6

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

2

2

2

1
This Paxillin antibody is un-conjugated
应用范围
153

59

41

40

37

24

22

19

15

14

12

6

2

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

原理

Rabbit IgG polyclonal antibody for Paxillin(PXN) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

序列

HEKDGKAYCR KDYFDMF

交叉反应（详细）

No cross reactivity with other proteins.

产品特性

Rabbit IgG polyclonal antibody for Paxillin(PXN) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: paxillin
Protein Name: Paxillin

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the C-terminal of human Paxillin(456-472aa HEKDGKAYCRKDYFDMF), identical to the related mouse and rat sequences.

亚型

IgG

Top Product

Discover our top product PXN Primary Antibody
anti-Paxillin (PXN) (AA 201-461) antibody
PXN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Paxillin (PXN) (AA 347-578) antibody
PXN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Paxillin (PXN) (AA 1-262) antibody
PXN 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-Paxillin (PXN) (AA 327-568) antibody
PXN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Paxillin (PXN) (AA 456-472) antibody
PXN 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Paxillin (PXN) (pTyr118) antibody
PXN 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Paxillin (PXN) (AA 1-262) antibody
PXN 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Paxillin (PXN) (pSer178) antibody
PXN 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Paxillin (PXN) (AA 28-45) antibody
PXN 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Paxillin (PXN) (pTyr88) antibody
PXN 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Thimerosal (Merthiolate), Sodium azide

注意事项

This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

有效期

12 months
Zhu, Liu, Li, Liu, Wang, Sun, Xiong, Jiang, Zheng, Hu: "Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility." in: Carcinogenesis, Vol. 35, Issue 8, pp. 1901-10, (2014) (PubMed).

Liu, Zhu, Xiong, Zheng, Hu, Qiu: "Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells." in: International journal of clinical and experimental pathology, Vol. 7, Issue 9, pp. 5750-61, (2014) (PubMed).

Zhong, Luo, Wang, Tang, Wu, Wang, Huang, Sun, Huang: "Effects of polydopamine functionalized titanium dioxide nanotubes on endothelial cell and smooth muscle cell." in: Colloids and surfaces. B, Biointerfaces, Vol. 116, pp. 553-60, (2014) (PubMed).
抗原

Paxillin (PXN)

别名

PXN (PXN 产品)

别名

AW108311 antibody, AW123232 antibody, Pax antibody, CG18061 antibody, CG18576 antibody, CG31794 antibody, CT40481 antibody, CT42454 antibody, DPaxillin antibody, DPxn antibody, DPxn37 antibody, Dmel\\CG31794 antibody, Dpax antibody, DpaxA antibody, PDLP antibody, dPax antibody, pax antibody, wu:fw71f12 antibody, PXN antibody, LOC100220858 antibody, paxillin antibody, Paxillin antibody, paxillin S homeolog antibody, paxillin a antibody, PXN antibody, Pxn antibody, Pax antibody, pxn.S antibody, pxna antibody, LOC696336 antibody, pxn antibody

背景

PXN(Paxillin) is a signal transduction adaptor protein discovered in 1990 in the laboratory of Keith Burridge. Salgia et al.(1995) mapped the paxillin gene to 12q24 using fluorescence in situ hybridization.The C-terminal region of paxillin contains four LIM domains that target paxillin to focal adhesions, it is presumed through a direct association with the cytoplasmic tail of beta-integrin. The N-terminal region of paxillin is rich in protein-protein interaction sites. The proteins that bind to paxillin are diverse and include protein tyrosine kinases, such as Src and FAK, structural proteins, such as vinculin and actopaxin, and regulators of actin organization, such as COOL/PIX and PKL/GIT. Paxillin is tyrosine-phosphorylated by FAK and Src upon integrin engagement or growth factor stimulation, creating binding sites for the adapter protein Crk.The paxillin protein contains 4 LIM domains, a proline-rich domain containing a consensus SH3-binding site, and 3 potential SH2-binding sites. On Northern blots, paxillin was expressed as a 3.7-kb mRNA in all tissues tested.

Synonyms: FLJ16691 antibody|FLJ23042 antibody|Paired box protein Pax 1 antibody|PAX 1 antibody|PAX1 antibody|PAXI_HUMAN antibody|Paxillin alpha antibody|Paxillin antibody|PXN antibody|PXN protein antibody

UniProt

P49023

途径

MAPK Pathway, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling