-
- 抗原 See all IBSP 抗体
- IBSP (Integrin-Binding Sialoprotein (IBSP))
-
抗原表位
- AA 305-324, C-Term
-
适用
- 小鼠, 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This IBSP antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Bone sialoprotein 2(IBSP) detection. Tested with WB, IHC-P in Mouse,Rat.
- 序列
- SYYKGHGYEG YEGQNYYYHQ
- 交叉反应 (详细)
- No cross reactivity with other proteins.
- 产品特性
-
Rabbit IgG polyclonal antibody for Bone sialoprotein 2(IBSP) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: integrin-binding sialoprotein
Protein Name: Bone sialoprotein 2 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bone Sialoprotein(305-324aa SYYKGHGYEGYEGQNYYYHQ), different from the related rat sequence by one amino acid.
- 亚型
- IgG
- Top Product
- Discover our top product IBSP Primary Antibody
-
-
- 应用备注
-
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Mouse, The detection limit for IBSP is approximately 2.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
-
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
-
-
Effect of kidney-reinforcing and marrow-beneficial Chinese medicine on bone metabolism-related factors following spinal cord injury in rats." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 485-491, (2016) (PubMed).
: "The osteogenic potential of mesoporous bioglasses/silk and non-mesoporous bioglasses/silk scaffolds in ovariectomized rats: in vitro and in vivo evaluation." in: PLoS ONE, Vol. 8, Issue 11, pp. e81014, (2014) (PubMed).
: "
-
Effect of kidney-reinforcing and marrow-beneficial Chinese medicine on bone metabolism-related factors following spinal cord injury in rats." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 485-491, (2016) (PubMed).
-
- 抗原
- IBSP (Integrin-Binding Sialoprotein (IBSP))
- 别名
- IBSP (IBSP 产品)
- 别名
- BNSP antibody, BSP antibody, BSP-II antibody, SP-II antibody, Bsp antibody, BSP II antibody, BSPII antibody, Bsp2 antibody, IBSP antibody, integrin binding sialoprotein antibody, integrin-binding sialoprotein antibody, integrin-binding sialoprotein S homeolog antibody, IBSP antibody, Ibsp antibody, ibsp.S antibody, ibsp antibody
- 背景
-
IBSP(integrin-binding sialoprotein) is also known as BSP. The protein encoded by this gene is a major structural protein of the bone matrix. Bone sialoprotein is an acidic glycoprotein of approximately 70 kD that undergoes extensive posttranslational modifications. It constitutes approximately 12 % of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.
Synonyms: BNSP antibody|Bone sialoprotein 2 antibody|Bone sialoprotein II antibody|BSP antibody|BSP II antibody|BSPII antibody|Cell binding sialoprotein antibody|Cell-binding sialoprotein antibody|IBSP antibody|Integrin binding sialoprotein antibody|Integrin-binding sialoprotein antibody|SIAL_HUMAN antibody|SPII antibody - UniProt
- Q61711
-