Splicing Factor 1 抗体 (N-Term)
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- 抗原 See all Splicing Factor 1 (SF1) 抗体
- Splicing Factor 1 (SF1)
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抗原表位
- AA 11-30, N-Term
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适用
- 人, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Splicing Factor 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 原理
- Rabbit IgG polyclonal antibody for Splicing factor 1(SF1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
- 序列
- DFPSKKRKRS RWNQDTMEQK
- 交叉反应 (详细)
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Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - 产品特性
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Rabbit IgG polyclonal antibody for Splicing factor 1(SF1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: splicing factor 1
Protein Name: Splicing factor 1 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human splicing factor 1(11-30aa DFPSKKRKRSRWNQDTMEQK), identical to the related rat and mouse sequences.
- 亚型
- IgG
- Top Product
- Discover our top product SF1 Primary Antibody
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
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- 抗原
- Splicing Factor 1 (SF1)
- 别名
- SF1 (SF1 产品)
- 背景
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SF1(splicing factor 1), also known as zinc finger protein 162(ZFM162), ZNF162, Transcription factor ZFM1, ZFM1, D11S636, Mammalian branch point-binding protein(BBP, mBBP), Zinc finger gene in MEN1 locus, is a protein that in humans is encoded by the SF1 gene. It is predicted 623-amino acid protein. RT-PCR was used to show expression in the thyroid gland, pancreas, adrenal gland, and ovary. All of the ZNF162 isoforms contain a KH domain, a sequence motif present in proteins playinig a major role in regulating cellular RNA metabolism.
Synonyms: BBP antibody|D11S636 antibody|Mammalian branch point-binding protein mBBP antibody|OTTHUMP00000069749 antibody|OTTHUMP00000069750 antibody|OTTHUMP00000069752 antibody|splicing factor 1 antibody|Transcription factor ZFM1 antibody|ZFM1 antibody|Zinc finger gene in MEN1 locus antibody|Zinc finger protein 162 antibody|ZNF162 antibody - UniProt
- Q15637
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location
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