FLI1 抗体 (N-Term)
(1 reference)-
- 抗原 See all FLI1 抗体
- FLI1 (Friend Leukemia Virus Integration 1 (FLI1))
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抗原表位
- AA 54-73, N-Term
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适用
- 人, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This FLI1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Friend leukemia integration 1 transcription factor(FLI1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- 序列
- QQEWINQPVR VNVKREYDHM
- 交叉反应 （详细）
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Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - 产品特性
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Rabbit IgG polyclonal antibody for Friend leukemia integration 1 transcription factor(FLI1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: Friend leukemia virus integration 1
Protein Name: Friend leukemia integration 1 transcription factor - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human FLI1(54-73aa QQEWINQPVRVNVKREYDHM), identical to the related rat and mouse sequences.
- 亚型
- IgG
- Top Product
- Discover our top product FLI1 Primary Antibody
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anti-Friend Leukemia Virus Integration 1 (FLI1) (AA 303-452) antibody
FLI1 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4F2A9 unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (AA 1-452) antibodyFLI1 适用: 人 WB, ELISA, IF, RNAi 宿主: 小鼠 Monoclonal 1F6 unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (AA 205-331) antibodyFLI1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (AA 1-452) antibodyFLI1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1G8 unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (N-Term) antibodyFLI1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 兔, 马, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (Internal Region) antibodyFLI1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (Internal Region) antibodyFLI1 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (AA 1-196) antibodyFLI1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (Internal Region) antibodyFLI1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (AA 1-100) antibodyFLI1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
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: "Endothelial Cell Infection by Guinea Pig Cytomegalovirus Is a Lytic or Persistent Infection Depending on Tissue Origin but Requires Viral Pentamer Complex and pp65 Tegument Protein." in: Journal of virology, Vol. 96, Issue 17, pp. e0083122, (2022) (PubMed).
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: "Endothelial Cell Infection by Guinea Pig Cytomegalovirus Is a Lytic or Persistent Infection Depending on Tissue Origin but Requires Viral Pentamer Complex and pp65 Tegument Protein." in: Journal of virology, Vol. 96, Issue 17, pp. e0083122, (2022) (PubMed).
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- 抗原
- FLI1 (Friend Leukemia Virus Integration 1 (FLI1))
- 别名
- FLI1 (FLI1 产品)
- 别名
- EWSR2 antibody, SIC-1 antibody, FLI1 antibody, fli antibody, Xfli antibody, fli1 antibody, Xfli1 antibody, fli-1 antibody, Xfli-1 antibody, MGC121593 antibody, Fli-1 antibody, Sic1 antibody, cb855 antibody, wu:fc45b11 antibody, xlfli antibody, Fli-1 proto-oncogene, ETS transcription factor antibody, Fli-1 proto-oncogene, ETS transcription factor S homeolog antibody, Friend leukemia integration 1 antibody, Fli-1 proto-oncogene, ETS transcription factor a antibody, Fli-1 proto-oncogene, ETS transcription factor L homeolog antibody, FLI1 antibody, Fli1 antibody, fli1.S antibody, fli1 antibody, fli1a antibody, fli1.L antibody
- 背景
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Friend leukemia integration 1 transcription factor, also known as Transcription factor ERGB or Proto-oncogene Fli-1 is a protein that in humans is encoded by the human FLI1 gene. The fli1 protooncogene encodes a member of the ETS family of winged helix-turn-helix transcription factors. This gene is mapped to 11q24.3. Fli-1 is activated through retroviral insertional mutagenesis in 90 % of F-MuLV-induced erythroleukemias. The constitutive activation of fli-1 in erythroblasts leads to a dramatic shift in the Epo/Epo-R signal transduction pathway, blocking erythroid differentiation, activating the Ras pathway, and resulting in massive Epo-independent proliferation of erythroblasts. These results suggest that Fli-1 overexpression in erythroblasts alters their responsiveness to Epo and triggers abnormal proliferation by switching the signaling event(s) associated with terminal differentiation to proliferation.
Synonyms: ERGB transcription factor antibody|Ewing Sarcoma breakpoint region 2 antibody|EWSR 2 antibody|EWSR2 antibody|FLI 1 antibody|FLI 1 proto oncogene antibody|FLI1 antibody|FLI1 EWS fusion gene antibody|FLI1 proto oncogene antibody|FLI1_HUMAN antibody|Friend leukemia integration 1 transcription factor antibody|Friend leukemia virus integration 1 antibody|Proto-oncogene Fli-1 antibody|SIC 1 antibody|SIC1 antibody|Transcription factor ERGB antibody|Viral integration region FLI1 antibody - UniProt
- Q01543
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