YBX1 抗体 (C-Term)
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- 抗原 See all YBX1 抗体
- YBX1 (Y Box Binding Protein 1 (YBX1))
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抗原表位
- AA 293-320, C-Term
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适用
- 人, 大鼠, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This YBX1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 原理
- Rabbit IgG polyclonal antibody for Nuclease-sensitive element-binding protein 1(YBX1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
- 序列
- ENPKPQDGKE TKAADPPAEN SSAPEAEQ
- 交叉反应 (详细)
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Nuclease-sensitive element-binding protein 1(YBX1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: Y box binding protein 1
Protein Name: Nuclease-sensitive element-binding protein 1 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human YB1 (293-320aa ENPKPQDGKETKAADPPAENSSAPEAEQ), identical to the related mouse and rat sequences.
- 亚型
- IgG
- Top Product
- Discover our top product YBX1 Primary Antibody
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for YB1 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- YBX1 (Y Box Binding Protein 1 (YBX1))
- 别名
- YBX1 (YBX1 产品)
- 别名
- BP-8 antibody, CSDA2 antibody, CSDB antibody, DBPB antibody, MDR-NF1 antibody, NSEP-1 antibody, NSEP1 antibody, YB-1 antibody, YB1 antibody, Byb1 antibody, Cbfa antibody, Dbpb antibody, Ef1a antibody, Msy1 antibody, Nsep1 antibody, Yb1 antibody, bp-8 antibody, csda2 antibody, csdb antibody, dbpb antibody, frgy1 antibody, mdr-nf1 antibody, nsep-1 antibody, nsep1 antibody, yb1 antibody, 1700102N10Rik antibody, C79409 antibody, EF1A antibody, MSY1 antibody, dbpB antibody, mYB-1a antibody, nuclease sensitive element binding protein 1 antibody, Y-box binding protein 1 antibody, Y box binding protein 1 antibody, Y-box binding protein 1 S homeolog antibody, nuclease-sensitive element-binding protein 1 antibody, Y box protein 1 antibody, nsep1 antibody, YBX1 antibody, Ybx1 antibody, ybx1.S antibody, LOC479011 antibody
- 背景
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YBX1(Y box binding protein 1), commonly referred to as "YB-1" by researchers, is a human protein. YB1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It has a molecular mass of 35,414 and contains 18 % basic residues and putative nuclear localization signals. The YBX1 gene is mapped on 1p34.2. Ybx1 was highly expressed in mouse erythroid myeloid lymphoid clone-1 (EML), a hematopoietic precursor cell line, but that it was downregulated in myeloid progenitors and in Gmcsf-treated EML cells during myeloid differentiation. Ybx1 was expressed at high levels in myeloid leukemic cells at different developmental stages. Knockdown of YBX1 in a human leukemic cell line inhibited proliferation ability, induced apoptosis, and induced megakaryocytic differentiation in response to arsenic trioxide treatment. YBX1 is downregulated during myeloid differentiation and aberrant YBX1 expression in leukemic cells may contribute to leukemia development by blocking differentiation.
Synonyms: BP 8 antibody|CBF-A antibody|CCAAT binding transcription factor I subunit A antibody|CCAAT-binding transcription factor I subunit A antibody|CSDA2 antibody|CSDB antibody|DBPB antibody|DNA binding protein B antibody|DNA-binding protein B antibody|EFI-A antibody| Enhancer factor I subunit A antibody|MDR NF1 antibody|MGC104858 antibody|MGC110976 antibody|MGC117250 antibody|NSEP 1 antibody|NSEP1 antibody|Nuclease sensitive element binding protein 1 antibody|Nuclease-sensitive element-binding protein 1 antibody|p50 antibody|Q15905 antibody|Y-box binding protein 1 antibody|Y-box transcription factor antibody|Y-box-binding protein 1 antibody|YB 1 antibody|YB-1 antibody|YBOX1_HUMAN antibody| YBX 1 antibody|YBX1 antibody - 基因ID
- 4904
- UniProt
- P67809
- 途径
- Regulation of Muscle Cell Differentiation
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