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- 抗原 See all ULK3 抗体
- ULK3 (Unc-51-Like Kinase 3 (ULK3))
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抗原表位
- AA 427-454, C-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ULK3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase ULK3(ULK3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- 序列
- HTEVQNLMAR AEYLKEQVKM RESRWEAD
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase ULK3(ULK3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: unc-51 like kinase 3
Protein Name: Serine/threonine-protein kinase ULK3 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at C-terminus of human ULK3 (427-454aa HTEVQNLMARAEYLKEQVKMRESRWEAD), different from the related mouse and rat sequences by four amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product ULK3 Primary Antibody
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anti-Unc-51-Like Kinase 3 (ULK3) (AA 166-177) antibody (Atto 390)
ULK3 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal Atto 390
anti-Unc-51-Like Kinase 3 (ULK3) (AA 166-177) antibody (HRP)ULK3 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal HRP
anti-Unc-51-Like Kinase 3 (ULK3) (AA 166-177) antibody (FITC)ULK3 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal FITC
anti-Unc-51-Like Kinase 3 (ULK3) (AA 166-177) antibody (PE)ULK3 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal PE
anti-Unc-51-Like Kinase 3 (ULK3) (AA 166-177) antibody (PerCP)ULK3 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal PerCP
anti-Unc-51-Like Kinase 3 (ULK3) (AA 166-177) antibody (Atto 594)ULK3 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal Atto 594
anti-Unc-51-Like Kinase 3 (ULK3) (AA 166-177) antibody (Atto 488)ULK3 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal Atto 488
anti-Unc-51-Like Kinase 3 (ULK3) (Internal Region) antibodyULK3 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Unc-51-Like Kinase 3 (ULK3) (AA 435-468), (C-Term) antibodyULK3 适用: 人, 小鼠, 大鼠 WB 宿主: 小鼠 Monoclonal 1404CT148-89-25 unconjugated
anti-Unc-51-Like Kinase 3 (ULK3) antibodyULK3 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- ULK3 (Unc-51-Like Kinase 3 (ULK3))
- 别名
- ULK3 (ULK3 产品)
- 别名
- zgc:162196 antibody, 1200015E14Rik antibody, unc-51 like kinase 3 antibody, unc-51 like kinase 3 L homeolog antibody, unc-51-like kinase 3 antibody, ULK3 antibody, ulk3 antibody, Ulk3 antibody, ulk3.L antibody
- 背景
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Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. In search of potential homologues to Drosophila and human Fu, people have cloned human serine/threonine kinase ULK3 and assessed its ability to regulate GLI transcription factors, mediators of SHH signaling. And ULK3 enhances endogenous and over-expressed GLI1 and GLI2 transcriptional activity in cultured cells, as assessed by GLI-luciferase reporter assay. Besides that, ULK3 alters subcellular localization of GLI1, as assessed by immunofluorescent staining and immunoblotting assays. It is showed that ULK3 is an autophosphorylated kinase and phosphorylates GLI proteins in vitro. Also, ULK3 catalytical activity is crucial for its function in SHH pathway. It is demonstrated that ULK3 is widely expressed and its expression is higher in a number of tissues where Shh signaling is known to be active. The data suggest that serine/threonine kinase ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins.
Synonyms: DKFZP434C131 antibody|FLJ90566 antibody|Serine/threonine-protein kinase ULK3 antibody|Ulk3 antibody|ULK3_HUMAN antibody|unc 51 like kinase 3 (C. elegans) antibody|Unc-51-like kinase 3 antibody - 基因ID
- 25989
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