UBA3 抗体 (C-Term)
Quick Overview for UBA3 抗体 (C-Term) (ABIN3043952)
抗原
See all UBA3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 409-448, C-Term
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原理
- Anti-UBE1C/UBA3 Antibody Picoband®
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序列
- KNRTLYLQSV TSIEERTRPN LSKTLKELGL VDGQELAVAD
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-UBE1C/UBA3 Antibody Picoband® (ABIN3043952). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human UBE1C, identical to the related mouse and rat sequences.
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亚型
- IgG
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anti-Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (AA 415-445), (C-Term) antibody
UBA3 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB2803 unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (Internal Region) antibodyUBA3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) antibodyUBA3 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (N-Term) antibodyUBA3 适用: 人, 小鼠, 大鼠, Cow, 犬, 斑马鱼, 马, Pig, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) antibodyUBA3 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (AA 234-463), (Catalytic Subunit) antibodyUBA3 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (AA 35-84) antibodyUBA3 适用: 人, 小鼠, 大鼠, Cow, 犬, 斑马鱼, 马, Pig, 兔, 小鸡, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (AA 1-229) antibodyUBA3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (N-Term) antibodyUBA3 适用: 人, 小鼠, 大鼠, Cow, 犬, 斑马鱼, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (C-Term) antibodyUBA3 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. "Entrez Gene: UBE1C ubiquitin-activating enzyme E1C (UBA3 homolog, yeast)". 2. Bohnsack, R. N., Haas, A. L. Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. J. Biol. Chem. 278: 26823-26830, 2003. 3. Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S (August 1998). "A new NEDD8-ligating system for cullin-4A". Genes Dev 12 (15): 2263-8. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))
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别名
- UBA3
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背景
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Synonyms: NEDD8-activating enzyme E1 catalytic subunit,6.3.2.-,NEDD8-activating enzyme E1C,Ubiquitin-activating enzyme E1C,Ubiquitin-like modifier-activating enzyme 3,Ubiquitin-activating enzyme 3,UBA3,UBE1C,
Tissue Specificity: Ubiquitously expressed. .
Background: NEDD8-activating enzyme E1 catalytic subunit is a protein that in humans is encoded by the UBA3 gene. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, an ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
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分子量
- 52 kDa
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基因ID
- 9039
抗原
-
