TFAM 抗体 (N-Term)
(2 references)-
- 抗原 See all TFAM 抗体
- TFAM (Transcription Factor A, Mitochondrial (TFAM))
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抗原表位
- AA 214-241, N-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TFAM antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Transcription factor A, mitochondrial(TFAM) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- 序列
- EMKSWEEQMI EVGRKDLLRR TIKKQRKY
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Transcription factor A, mitochondrial(TFAM) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: transcription factor A, mitochondrial
Protein Name: Transcription factor A, mitochondrial - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human mtTFA (214-241aa EMKSWEEQMIEVGRKDLLRRTIKKQRKY), different from the related mouse and rat sequences by five amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product TFAM Primary Antibody
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anti-Transcription Factor A, Mitochondrial (TFAM) (N-Term) antibody
TFAM 适用: 人, 小鼠, 兔, Cow, 犬, 豚鼠, 马, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) (AA 43-243) antibodyTFAM 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) (AA 1-220) antibodyTFAM 适用: 人 WB, IHC, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) (AA 1-246) antibodyTFAM 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) antibodyKO Validated TFAM 适用: 人 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) (AA 227-246), (C-Term) antibodyTFAM 适用: 人 WB, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) (AA 216-246), (C-Term) antibodyTFAM 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB27631 unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) (AA 1-246) antibodyTFAM 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) antibodyTFAM 适用: 人 WB, IHC, IF, ChIP 宿主: 兔 Monoclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) (AA 216-246) antibodyTFAM 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for mtTFA is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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: "Roles of mitochondrial transcription factor A and microRNA‑590‑3p in the development of colon cancer." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 5475-5480, (2017) (PubMed).
: "Roles of mitochondrial transcription factor A and microRNA-590-3p in the development of bladder cancer." in: Oncology letters, Vol. 6, Issue 2, pp. 617-623, (2013) (PubMed).
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: "Roles of mitochondrial transcription factor A and microRNA‑590‑3p in the development of colon cancer." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 5475-5480, (2017) (PubMed).
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- 抗原
- TFAM (Transcription Factor A, Mitochondrial (TFAM))
- 别名
- TFAM (TFAM 产品)
- 别名
- tcf6 antibody, mttf1 antibody, mttfa antibody, tcf6l1 antibody, tcf6l2 antibody, tcf6l3 antibody, mttfa-A antibody, xl-mtTFA antibody, MGC153358 antibody, zgc:153358 antibody, MTTF1 antibody, MTTFA antibody, TCF6 antibody, TCF6L1 antibody, TCF6L2 antibody, TCF6L3 antibody, AI661103 antibody, Hmgts antibody, mtTFA antibody, tsHMG antibody, Mttfa antibody, transcription factor A, mitochondrial antibody, transcription factor A, mitochondrial L homeolog antibody, TFAM antibody, tfam.L antibody, tfam antibody, Tfam antibody
- 背景
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TFAM (Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse mitochondrial transcription factor A gene (Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.
Synonyms: anscription factor 6-like 1 antibody|Mitochondrial transcription factor 1 antibody| mitochondrial transcription factor A antibody|MtTF1 antibody|mtTFA antibody|TCF 6 antibody| TCF-6 antibody|TCF6 antibody|TCF6L1 antibody|TCF6L2 antibody|TCF6L3 antibody|TFAM antibody| TFAM_HUMAN antibody|Transcription factor 6 antibody|Transcription factor 6 like 2 (mitochondrial transcription factor) antibody|Transcription factor 6 like 2 antibody| Transcription factor 6-like 2 antibody|transcription factor 6-like 3 antibody|Transcription factor A, mitochondrial antibody|Transcription factor A, mitochondrial precursor antibody - 基因ID
- 7019
- UniProt
- Q00059
- 途径
- Chromatin Binding
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