MCM7 抗体 (AA 526-719)
Quick Overview for MCM7 抗体 (AA 526-719) (ABIN3043877)
抗原
See all MCM7 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 526-719
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原理
- Anti-MCM7 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-MCM7 Antibody Picoband® (ABIN3043877). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human MCM7 recombinant protein (Position: D526-V719). Human MCM7 shares 94% amino acid (aa) sequence identity with mouse MCM7.
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亚型
- IgG
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anti-Minichromosome Maintenance Complex Component 7 (MCM7) (AA 1-389) antibody
MCM7 适用: 人 WB, IF, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 6C2 unconjugated
anti-Minichromosome Maintenance Complex Component 7 (MCM7) (AA 360-719) antibodyMCM7 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Complex Component 7 (MCM7) antibodyMCM7 适用: 人 WB, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Complex Component 7 (MCM7) (Internal Region) antibodyMCM7 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Minichromosome Maintenance Complex Component 7 (MCM7) (AA 195-319) antibodyMCM7 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal rMCM7-1468 unconjugated Recombinant Antibody
anti-Minichromosome Maintenance Complex Component 7 (MCM7) (AA 195-319) antibodyMCM7 适用: 人 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal MCM7-1467 unconjugated
anti-Minichromosome Maintenance Complex Component 7 (MCM7) (AA 195-319) antibodyMCM7 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal MCM7-2832R unconjugated Recombinant Antibody
anti-Minichromosome Maintenance Complex Component 7 (MCM7) (AA 195-319) antibodyMCM7 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal MCM7-1466 unconjugated
anti-Minichromosome Maintenance Complex Component 7 (MCM7) (AA 1-719) antibodyMCM7 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Minichromosome Maintenance Complex Component 7 (MCM7) (AA 1-719) antibodyMCM7 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry, 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. 2. Nakatsuru, S., Sudo, K., Nakamura, Y. Isolation and mapping of a human gene (MCM2) encoding a product homologous to yeast proteins involved in DNA replication.Cytogenet. Cell Genet. 68: 226-230, 1995. 3. Petrocca, F., Visone, R., Onelli, M. R., Shah, M. H., Nicoloso, M. S., de Martino, I., Iliopoulos, D., Pilozzi, E., Liu, C.-G., Negrini, M., Cavazzini, L., Volinia, S., Alder, H., Ruco, L. P., Baldassarre, G., Croce, C. M., Vecchione, A. E2F1-regulated microRNAs impair TGF-beta-dependent cell-cycle arrest and apoptosis in gastric cancer. Cancer Cell 13: 272-286, 2008. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))
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别名
- MCM7
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背景
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Synonyms: DNA replication licensing factor MCM7,3.6.4.12,CDC47 homolog,P1.1-MCM3,MCM7,CDC47, MCM2,
Tissue Specificity: Expressed in prostate.
Background: MCM7 (Minichromosome Maintenance, s. Cerevisiae, homolog of, 7), also called CDC47, FORMERLY, is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM7 gene is mapped to 7q22.1. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle. The MCM7 gene contains 15 exons. The miRNAs MIR106B, MIR93, and MIR25 are clustered in a 5-prime to 3-prime orientation within intron 13. It has been found that MCM7 and the precursors of microRNAs (miRNAs) MIR106B, MIR93, and MIR25, all of which arise from intron 13 of the MCM7 gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors.
Sequence Similarities: Belongs to the MCM family.
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分子量
- 81 kDa
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基因ID
- 4176
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UniProt
- P33993
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途径
- DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
抗原
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