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Dihydrofolate Reductase 抗体 (AA 2-187)

DHFR 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3043286
发货至: 中国
  • 抗原 See all Dihydrofolate Reductase (DHFR) 抗体
    Dihydrofolate Reductase (DHFR)
    抗原表位
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    AA 2-187
    适用
    • 31
    • 19
    • 16
    • 1
    • 1
    人, 小鼠, 大鼠
    宿主
    • 28
    • 6
    • 1
    克隆类型
    • 28
    • 7
    多克隆
    标记
    • 24
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Dihydrofolate Reductase antibody is un-conjugated
    应用范围
    • 22
    • 13
    • 10
    • 10
    • 7
    • 3
    • 3
    • 2
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    原理
    Rabbit IgG polyclonal antibody for Dihydrofolate reductase(DHFR) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    交叉反应 (详细)
    No cross reactivity with other proteins.
    产品特性
    Rabbit IgG polyclonal antibody for Dihydrofolate reductase(DHFR) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Gene Name: dihydrofolate reductase
    Protein Name: Dihydrofolate reductase
    纯化方法
    Immunogen affinity purified.
    免疫原
    E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFR shares 90% amino acid (aa) sequence identity with both mouse and rat DHFR.
    亚型
    IgG
    Top Product
    Discover our top product DHFR Primary Antibody
  • 应用备注
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat
    Notes: Reacts with: human, mouse, rat
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Tian, Zhang, Ge, Xiao, Lu, Fu, Liu, Sun: "MicroRNA-205 suppresses proliferation and promotes apoptosis in laryngeal squamous cell carcinoma." in: Medical oncology (Northwood, London, England), Vol. 31, Issue 1, pp. 785, (2013) (PubMed).

  • 抗原
    Dihydrofolate Reductase (DHFR)
    别名
    DHFR (DHFR 产品)
    别名
    DHFR antibody, F10_orf160 antibody, ECK0049 antibody, JW0047 antibody, tmrA antibody, cb595 antibody, zgc:86637 antibody, DDBDRAFT_0187132 antibody, DDBDRAFT_0230113 antibody, DDB_0187132 antibody, DDB_0230113 antibody, CG14887 antibody, Dmel\\CG14887 antibody, dhfr antibody, DHFRP1 antibody, DYR antibody, 8430436I03Rik antibody, AA607882 antibody, AI662710 antibody, AW555094 antibody, Dhfr1 antibody, confers resistance to sulfonamides antibody, dihydrofolate reductase antibody, dihydrofolate reductase DhfR antibody, Dihydrofolate reductase antibody, dhfr antibody, DHFR antibody, dhfR antibody, folA antibody, dfrA antibody, LOC707382 antibody, CNJ01940 antibody, DDB_G0286755 antibody, Dhfr antibody, Mesil_0096 antibody, Arch_0408 antibody, Fbal_3367 antibody, Ilyop_0482 antibody, Lbys_2007 antibody, Riean_0733 antibody, Mrub_1855 antibody, Poras_0766 antibody, Halhy_3552 antibody, Mesop_3614 antibody, LOC609048 antibody
    背景
    Dihydrofolate reductase, or DHFR, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. In humans, the DHFR enzyme is encoded by the DHFR gene. It is found in the q11→q22 region of chromosome 5. What's more, DHFR belongs to the dihydrofolate reductase family, and it converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. DHFR is the key enzyme in folate metabolism. In addition, DHFR catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

    Synonyms: DHFR antibody|DHFRP1 antibody|Dihydrofolate reductase antibody|DYR antibody|DYR_HUMAN antibody|EC 1.5.1.3 antibody
    基因ID
    1719
    UniProt
    P00374
    途径
    Mitotic G1-G1/S Phases
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