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CD20 抗体 (AA 1-261)

Name: CD20 抗体 (AA 1-261)
SKU: ABIN3042388
Availability: OutOfStock

MS4A1 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

Flow Cytometry analysis of U937 cells using anti-CD20 antibody . (CD20 抗体 (AA 1-261))

FACS

Anti-CD20 Picoband antibody, IHC(P): Human Tonsil Tissue (CD20 抗体 (AA 1-261))

IHC

Western blot analysis of CD20 using anti-CD20 antibody . (CD20 抗体 (AA 1-261))

4 images

(2 references)

产品编号 ABIN3042388

发货至: 中国

Datasheet as PDF

抗原 See all CD20 (MS4A1) 抗体

CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
抗原表位
17

16

16

8

6

5

5

4

3

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 1-261
适用
254

63

28

23

18

17

11

7

6

4

2

1
人
宿主
183

97

4

2
兔
Compatible Secondaries
克隆类型
202

82

1
多克隆
标记
107

21

19

18

14

7

6

6

6

6

6

6

5

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD20 antibody is un-conjugated
应用范围
209

91

76

52

47

33

25

19

15

15

14

13

11

11

6

5

3

2

2

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

原理

Rabbit IgG polyclonal antibody for B-lymphocyte antigen CD20(MS4A1) detection. Tested with WB, IHC-P, FCM in Human.

交叉反应（详细）

No cross reactivity with other proteins.

产品特性

Rabbit IgG polyclonal antibody for B-lymphocyte antigen CD20(MS4A1) detection. Tested with WB, IHC-P, FCM in Human.
Gene Name: membrane-spanning 4-domains, subfamily A, member 1
Protein Name: B-lymphocyte antigen CD20

纯化方法

Immunogen affinity purified.

免疫原

E.coli-derived human CD20 recombinant protein (Position: M1-D261). Human CD20 shares 75% amino acid (aa) sequence identity with mouse CD20.

亚型

IgG

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anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody
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anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibody
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anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody
MS4A1 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal rIGEL-773 unconjugated Recombinant Antibody

anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody
MS4A1 适用: 人, Cow FACS, IP 宿主: 小鼠 Monoclonal MEM-97 unconjugated

anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody
MS4A1 适用: 人, Cow FACS, IP 宿主: 小鼠 Monoclonal MEM-97 unconjugated

anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibody
MS4A1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 210-297) antibody
MS4A1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5C11 unconjugated

应用备注

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD20 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Flow Cytometry: Concentration:1-3 μg/1x106 cells, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Ni, Chen, Zeng, Pei, Du, Tang, Wang, Hu, Zhu, He, Wei, Wang, Ning, Wang, Wang, Ma, Chen, Sun, Tang, Wang, Wang: "In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures." in: Cell research, Vol. 25, Issue 7, pp. 785-800, (2016) (PubMed).

Yan, Fan, Bai, Hou, Li, Tong: "Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway." in: PLoS ONE, Vol. 11, Issue 2, pp. e0148536, (2016) (PubMed).
抗原

CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

别名

MS4A1 (MS4A1 产品)

别名

B1 antibody, Bp35 antibody, CD20 antibody, CVID5 antibody, LEU-16 antibody, MS4A2 antibody, S7 antibody, AA960661 antibody, Cd20 antibody, Ly-44 antibody, Ms4a2 antibody, MS4A1 antibody, bp35 antibody, cd20 antibody, ms4a2 antibody, leu-16 antibody, ms4a4c antibody, cd20-like antibody, membrane spanning 4-domains A1 antibody, membrane-spanning 4-domains, subfamily A, member 1 antibody, B-lymphocyte antigen CD20 antibody, MS4A1 antibody, Ms4a1 antibody, ms4a1 antibody, LOC101694281 antibody

背景

CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What's more, this protein may be involved in the regulation of B-cell activation and proliferation.

Synonyms: APY antibody|ATOPY antibody|B lymphocyte antigen CD20 antibody|B Lymphocyte Cell Surface Antigen B1 antibody|B-lymphocyte antigen CD20 antibody|B-lymphocyte cell-surface antigen B1 antibody|B-lymphocyte surface antigen B1 antibody|B1 antibody|Bp 35 antibody|Bp35 antibody|CD 20 antibody|CD20 antibody|CD20 antigen antibody|CD20 receptor antibody|CD20_HUMAN antibody|Fc epsilon receptor I beta chain antibody|Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody|FCER1B antibody|FCERI antibody|High affinity immunoglobulin epsilon receptor subunit beta antibody|IgE Fc receptor subunit beta antibody|IGEL antibody|IGER antibody|IGHER antibody|LEU16 antibody|LEU16 antibody|leukocyte surface antigen Leu 16 antibody|Leukocyte surface antigen Leu-16 antibody|Ly44 antibody|Membrane spanning 4 domains subfamily A member 2 antibody|Membrane-spanning 4-domains subfamily A member 1 antibody|MS4A1 antibody

基因ID

931

UniProt

P11836