14-3-3 sigma/SFN 抗体 (AA 140-156)
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- 抗原 See all 14-3-3 sigma/SFN (SFN) 抗体
- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
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抗原表位
- AA 140-156
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This 14-3-3 sigma/SFN antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 纯化方法
- Antigen affinity
- 免疫原
- Amino acids 140-156 (KKRIIDSARSAYQEAMD) were used as the immunogen for this 14-3-3 sigma antibody (100% homologous in human, mouse and rat).
- 亚型
- IgG
- Top Product
- Discover our top product SFN Primary Antibody
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- 应用备注
- The stated application concentrations are suggested starting amounts. Titration of the 14-3-3 sigma antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
- 限制
- 仅限研究用
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- 缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
- 储存条件
- -20 °C
- 储存方法
- After reconstitution, the 14-3-3 sigma antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- 抗原
- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
- 别名
- 14-3-3 sigma (SFN 产品)
- 别名
- YWHAS antibody, Er antibody, Mme1 antibody, Ywhas antibody, stratifin antibody, SFN antibody, Sfn antibody
- 背景
- Stratifin (SFN), also known as 14-3-3 sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al.(1993) obtained a peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein called stratifin was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.
- UniProt
- P31947
- 途径
- p53 Pathway, Myometrial Relaxation and Contraction
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