Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
纯化方法
Antigen affinity
免疫原
An amino acid sequence from the N-terminus of human Smad2 (DQWDTTGLYSFSEQTRSLD) was used as the immunogen for this Smad2 antibody (100% homologous in human, mouse and rat).
The stated application concentrations are suggested starting points. Titration of the Smad2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
限制
仅限研究用
缓冲液
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
储存条件
-20 °C
储存方法
After reconstitution, the Smad2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
抗原
SMAD2
(SMAD, Mothers Against DPP Homolog 2 (SMAD2))
SMAD family member 2, also known as MADR2, and MADH2 (Mothers against decapentaplegic homolog 2), is a protein that in humans is encoded by the SMAD2 gene. It belongs to the SMAD family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene 'Sma.' The gene has been mapped to 18q21.1, a region which is frequently deleted in colorectal cancers. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.