The stated application concentrations are suggested starting points. Titration of the SIRT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
限制
仅限研究用
缓冲液
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
储存条件
-20 °C
储存方法
After reconstitution, the SIRT2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Sirtuin 2 is a predominantly cytoplasmic protein that colocalizes with microtubules. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT2 is mapped to 19q13.2. It is able to cause radioactivity to be transferred from (32P) NAD to bovine serum albumin (BSA). According to researches, it can act as a tubulin deacetylase. SIRT2 was found to deacetylate lys40 of alpha-tubulin both in vitro and in vivo. It is also a regulator of mitotic progression that acts downstream from CDC14B in a pathway regulating mitotic exit or subsequent cytokinesis. Additionally, SIRT2 is an important regulator of programmed necrosis.