The stated application concentrations are suggested starting amounts. Titration of the SPARC antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
限制
仅限研究用
缓冲液
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
储存条件
-20 °C
储存方法
After reconstitution, the SPARC antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Secreted protein acidic and rich in cysteine, also known as Osteonectin, is a protein that in humans is encoded by the SPARC gene. The human gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus(domain I), 2) a cysteine- rich(domain II), 3) a hydrophilic region(domain III) and 4) an EF hand motif at the carboxy terminus region(domain IV). SPARC is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. It also shows affinity for collagen in addition to bone mineral calcium. A correlation between SPARC over expression and ampullary cancers and chronic pancreatitis has been found.