Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
纯化方法
Antigen affinity
免疫原
An amino acid sequence from the C-terminus of mouse Regulator of G Protein Signaling 3 (DSYTREHTKENLQSITR) was used as the immunogen for this RGS3 antibody (100% rat homology).
The stated application concentrations are suggested starting amounts. Titration of the RGS3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
限制
仅限研究用
缓冲液
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
储存条件
-20 °C
储存方法
After reconstitution, the RGS3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
RGS3 antibody, MGC115680 antibody, C2PA antibody, RGP3 antibody, 4930506N09Rik antibody, C2PA-RGS3 antibody, C2pa antibody, PDZ-RGS3 antibody, RGS3S antibody, SRB-RGS antibody, regulator of G protein signaling 3 antibody, regulator of G-protein signaling 3 L homeolog antibody, regulator of G-protein signaling 3 antibody, RGS3 antibody, rgs3.L antibody, Rgs3 antibody
背景
Regulator of G Protein Signaling 3 is a protein that in humans is encoded by the RGS3 gene. Chatterjee et al.(1997) studied RGS3, the largest member of the RGS family then identified. Mouse Pdz-Rgs3 can mediate signaling from the ephrin-B cytoplasmic tail. By radiation hybrid mapping, Chatterjee et al.(1997) assigned the gene to chromosome 9q31-q33. Lu et al.(2001) showed that Sdf1, a chemokine with a G protein-coupled receptor, and Bdnf are chemoattractants for cerebellar granule cells, and that Sdf1 chemoattraction is selectively inhibited by soluble ephrin-B receptor. This inhibition could be blocked by a truncated Pdz-Rgs3 protein lacking the RGS domain.