The stated application concentrations are suggested starting points. Titration of the CXCL4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
限制
仅限研究用
缓冲液
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
储存条件
-20 °C
储存方法
After reconstitution, the CXCL4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Chemokine (C-X-C motif) ligand 4, is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. It belongs to the CXC chemokine family. The gene contains 3 exons spanning approximately 1 kb. It is encoded on a 10-kb EcoRI fragment of genomic DNA. By in situ hybridization, the gene is mapped to chromosome 4q12-q21. CXCL4s major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, the cytokine probably has a role in inflammation and wound repair.