Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
纯化方法
Antigen affinity
免疫原
An amino acid sequence from the C-terminus of human HOXA3 (HHPSQGRIQEAPKLTHL) was used as the immunogen for this HOXA3 antibody (100% homologous in human, mouse and rat).
The stated application concentrations are suggested starting amounts. Titration of the HOXA3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
限制
仅限研究用
缓冲液
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
储存条件
-20 °C
储存方法
After reconstitution, the HOXA3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Homeobox A3 is a protein that in humans is encoded by the HOXA3 gene, which is also part of the A cluster on chromosome 7. The gene encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Its cytogenetic location is 7q15.2. In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. HOXA3 protein is able to complement Hoxd3 deficiency when expressed in the context of the Hoxd3 allele.