The stated application concentrations are suggested starting points. Titration of the Caspase-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
限制
仅限研究用
缓冲液
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
储存条件
-20 °C
储存方法
After reconstitution, the Caspase-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Caspase-1, an apoptosis-related cysteine protease, is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL-18, and IL-33 precursor proteins. It was purified from the cytosol of the THP monocytic cell line and found that the active protease was made up of two peptides, which they called p20 and p10 based on their apparent molecular masses by SDS-PAGE. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The CASP1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal metaphase chromosomes. Caspase-1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.