AQP2 抗体 (AA 245-260)
Quick Overview for AQP2 抗体 (AA 245-260) (ABIN3030003)
抗原
See all AQP2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 245-260
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纯化方法
- Antigen affinity
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免疫原
- Amino acids 245-260 (DWEEREVRRRQSVELH) were used as the immunogen for this AQP2 antibody (100% homologous in human, mouse and rat).
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亚型
- IgG
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anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody
AQP2 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p), ICC, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-271) antibodyAQP2 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 147-271) antibodyAQP2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 149-271) antibodyAQP2 适用: 人 ELISA 宿主: 小鼠 Monoclonal 7H8B5 unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (pSer261) antibodyAQP2 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (C-Term) antibodyAQP2 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 101-200) antibodyAQP2 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (pSer269) antibodyAQP2 适用: 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (C-Term) antibody (HRP)AQP2 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal HRP
anti-Aquaporin 2 (Collecting Duct) (AQP2) (C-Term) antibody (FITC)AQP2 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal FITC
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应用备注
- The stated application concentrations are suggested starting amounts. Titration of the AQP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- After reconstitution, the AQP2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
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别名
- AQP2 Aquaporin 2
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背景
- Aquaporin 2 also called Aquaporin-CD, is found in the apical cell membranes of the kidneys collecting duct principal cells and in intracellular vesicles located throughout the cell. The AQP2 gene is mapped to chromosome 12q13, very close to the site of major intrinsic protein by situ hybridization. Investigators suggest that a defect in the gene is the basis of the autosomal form of nephrogenic diabetes insipidus. The functional expression and the limited localization suggested that AQP2 is the vasopressin-regulated water channel. Using rat kidney slices and porcine kidney cells stably expressing rat Aquaporin 2, trafficking can be stimulated by cAMP-independent pathways that utilize nitric oxide(NO). The NO donors sodium nitroprusside(SNP) and NONOate and the NO synthase substrate L-arginine mimicked the effect of vasopressin(VP), stimulating relocation of Aquaporin 2 from cytoplasmic vesicles to the apical plasma membrane. SNP increased intracellular cGMP rather than cAMP, and exogenous cGMP stimulated AQP2 membrane insertion. Atrial natriuretic factor, which signals via cGMP, also stimulated AQP2 translocation. Expression in kidney connecting tubules is sufficient for survival and that expression in collecting ducts is required to regulate body water balance. The S256L substitution in the cytoplasmic tail of the protein prevented phosphorylation at S256 and the subsequent accumulation of Aquaporin 2 on the apical membrane of the collecting duct principal cells.
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UniProt
- P41181
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途径
- Response to Water Deprivation
抗原
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