Vinculin 抗体 (AA 173-188)
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- 抗原 See all Vinculin (VCL) 抗体
- Vinculin (VCL)
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抗原表位
- AA 173-188
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Vinculin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Antigen affinity
- 免疫原
- Amino acids 173-188 (KMIDERQQELTHQEHR) were used as the immunogen for this Vinculin antibody (100% homologous in human, mouse and rat).
- 亚型
- IgG
- Top Product
- Discover our top product VCL Primary Antibody
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anti-Vinculin (VCL) (AA 1-1066) antibody
VCL 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C6-1B5 unconjugated
anti-Vinculin (VCL) (AA 879-1064) antibodyVCL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vinculin (VCL) (AA 903-937) antibodyVCL 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB55983 unconjugated
anti-Vinculin (VCL) (AA 1020-1134) antibodyVCL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Vinculin (VCL) (AA 634-668) antibodyVCL 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB55982 unconjugated
anti-Vinculin (VCL) (AA 174-322) antibodyVCL 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal VCL-2572 unconjugated
anti-Vinculin (VCL) (AA 1-1066) antibodyVCL 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3F8-1D4 unconjugated
anti-Vinculin (VCL) (AA 174-322) antibodyVCL 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal VCL-2575 unconjugated
anti-Vinculin (VCL) (AA 767-1066) antibodyVCL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vinculin (VCL) antibodyVCL 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
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- 应用备注
- The stated application concentrations are suggested starting points. Titration of the Vinculin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
- 限制
- 仅限研究用
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- 缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
- 储存条件
- -20 °C
- 储存方法
- After reconstitution, the Vinculin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- 抗原
- Vinculin (VCL)
- 别名
- Vinculin (VCL 产品)
- 别名
- CMD1W antibody, CMH15 antibody, MV antibody, MVCL antibody, VCL antibody, im:6902790 antibody, hm:zehn2160 antibody, DDBDRAFT_0186762 antibody, DDBDRAFT_0232320 antibody, DDB_0186762 antibody, DDB_0232320 antibody, 9430097D22 antibody, AA571387 antibody, AI462105 antibody, AW545629 antibody, CG3299 antibody, CT11081 antibody, Dmel\\CG3299 antibody, Dmvincp antibody, EG:103B4.1 antibody, Vin antibody, Vin2EF antibody, vinc antibody, LOC100228062 antibody, vcl-A antibody, vinculin antibody, vinculin a antibody, vinculin A antibody, Vinculin antibody, vinculin S homeolog antibody, VCL antibody, vcla antibody, vinA antibody, Vcl antibody, Vinc antibody, CpipJ_CPIJ013873 antibody, Bm1_37935 antibody, vcl antibody, vcl.S antibody
- 背景
- In mammalian cells, Vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. By linkage studies in a 3-generation family, Mulligan et al.(1992) mapped the VCL gene to chromosome 10q22.1-q23, distal to D10S22. It is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is though to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Multiple alternatively-spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. The human protein exhibits a greater-than-95 % sequence identity to the chicken protein. Vinculin is a 117- kDa cytoskeletal protein with 1066 amino acids. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. It has a globular head domain that contains binding sites for talin and alpha-actinin as well as a tyrosine phosphorylation site, while the tail region contains binding sites for F-actin, paxillin, and lipids.
- UniProt
- P18206
- 途径
- Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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