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Neurofilament 抗体

适用: 人, 大鼠, 小鼠, 小鸡, Pig FACS, IHC (p) 宿主: 小鼠 Monoclonal RT-97-NR-4 unconjugated
产品编号 ABIN3026883
发货至: 中国
  • 抗原 See all Neurofilament products
    Neurofilament
    适用
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    人, 大鼠, 小鼠, 小鸡, Pig
    宿主
    • 21
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    小鼠
    克隆类型
    • 21
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    单克隆
    标记
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    This Neurofilament antibody is un-conjugated
    应用范围
    • 17
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    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    产品特性
    This mAb reacts with a 200 kDa and 68 kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
    纯化方法
    Protein G affinity chromatography
    免疫原
    Triton-X 100 insoluble proteins of rat brain (RT-97) and Neurofilaments from porcine spinal cord (NR-4) were used as the immunogen for the Neurofilament antibody cocktail.
    克隆位点
    RT-97-NR-4
    亚型
    IgG
  • 应用备注
    Optimal dilution of the Neurofilament antibody cocktail should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    储存液
    Azide free
    储存条件
    4 °C,-20 °C
    储存方法
    Store the Neurofilament antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    Neurofilament
    Abstract
    Neurofilament 产品
    背景
    This mAb reacts with a 200  kDa and 68  kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68  kDa (NF-L), 160  kDa (NF-M) and 200  kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
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