Keratin 10/13 抗体
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- 抗原
- Keratin 10/13
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适用
- 人, Cat
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 产品特性
- This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this mAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10 + 13 antibody.
- 克隆位点
- DE-K13
- 亚型
- IgG2a kappa
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anti-Keratin 10/13 antibody
适用: 人, Cat, 斑马鱼 WB, FACS, IHC (p), IHC, IHC (fro), ICC 宿主: 小鼠 Monoclonal DE-K13 unconjugated
anti-Keratin 10/13 antibody适用: 人, Cat WB, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal DE-K13 unconjugated
anti-Keratin 10/13 antibody适用: 人, Cat WB, FACS, IHC (p), IHC, IHC (fro), ICC 宿主: 小鼠 Monoclonal DE-K13 unconjugated
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- 应用备注
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Optimal dilution of the Cytokeratin 10 + 13 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.25-0.5 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Cytokeratin 10 + 13 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Keratin 10/13
- 别名
- Cytokeratin 10 + 13
- 背景
- This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this mAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
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