Actin, Muscle Specific 抗体
适用: 人, 兔, 大鼠
FACS, IF, IHC (p)
宿主: 小鼠
Monoclonal
SPM160
unconjugated
1 image
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- 抗原 See all Actin, Muscle Specific products
- Actin, Muscle Specific
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适用
- 人, 兔, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Actin, Muscle Specific antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 产品特性
- This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotype of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Human myocardium extract was used as the immunogen for this Muscle Specific Actin antibody.
- 克隆位点
- SPM160
- 亚型
- IgG1 kappa
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- 应用备注
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The optimal dilution of the Muscle Specific Actin antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1) - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Muscle Specific Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Actin, Muscle Specific
- 别名
- Muscle Specific Actin (Actin, Muscle Specific 产品)
- 背景
- This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotype of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
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