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CD63 抗体

CD63 适用: 人, 小鼠 FACS, WB, IF, IHC (p) 宿主: 小鼠 Monoclonal MX-49-129-5 unconjugated
产品编号 ABIN3026282
发货至: 中国
  • 抗原 See all CD63 抗体
    CD63
    适用
    • 128
    • 47
    • 10
    • 3
    • 2
    • 1
    人, 小鼠
    宿主
    • 75
    • 65
    • 4
    • 2
    • 1
    小鼠
    克隆类型
    • 98
    • 47
    • 2
    单克隆
    标记
    • 73
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    • 2
    • 2
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    This CD63 antibody is un-conjugated
    应用范围
    • 92
    • 64
    • 52
    • 44
    • 36
    • 28
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    • 12
    • 5
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    Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Protein G affinity chromatography
    免疫原
    Full length human CD63 was used as the immunogen for this antibody.
    克隆位点
    MX-49-129-5
    亚型
    IgG1 kappa
    Top Product
    Discover our top product CD63 Primary Antibody
  • 应用备注
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    储存液
    Azide free
    储存条件
    4 °C,-20 °C
    储存方法
    Store the CD63 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    CD63
    别名
    CD63 (CD63 产品)
    背景
    The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
    基因ID
    967
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