Calprotectin 抗体
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- 抗原 See all Calprotectin (S100A8/A9) 抗体
- Calprotectin (S100A8/A9)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Calprotectin antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- PEG precipitation
- 免疫原
- Recombinant human protein was used as the immunogen for the Calprotectin antibody.
- 克隆位点
- CPT-1028
- 亚型
- IgM kappa
- Top Product
- Discover our top product S100A8/A9 Primary Antibody
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anti-Calprotectin (S100A8/A9) (AA 1-114) antibody
S100A8/A9 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calprotectin (S100A8/A9) (AA 1-113) antibodyS100A8/A9 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calprotectin (S100A8/A9) (AA 1-114) antibodyS100A8/A9 适用: 人 IHC, WB, IP, ICC 宿主: 小鼠 Monoclonal C6 unconjugated
anti-Calprotectin (S100A8/A9) antibodyS100A8/A9 适用: 人 IHC, ELISA, WB 宿主: 小鼠 Monoclonal 69-6-1 unconjugated
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- 应用备注
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Optimal dilution of the Calprotectin antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Formalin-fixed): 0.5-1 μg/mL for 30 min at RT (1),Not suitable for frozen tissues
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Calprotectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Calprotectin (S100A8/A9)
- 别名
- Calprotectin (S100A8/A9 产品)
- 别名
- 60B8AG antibody, CAGB antibody, CFAG antibody, CGLB antibody, L1AG antibody, LIAG antibody, MAC387 antibody, MIF antibody, MRP14 antibody, NIF antibody, P14 antibody, Mrp14 antibody, MRP-14 antibody, p14 antibody, 60B8Ag antibody, AW546964 antibody, BEE22 antibody, Cagb antibody, GAGB antibody, L1Ag antibody, S100 calcium binding protein A9 antibody, S100 calcium binding protein A9 (calgranulin B) antibody, S100A9 antibody, S100a9 antibody
- 背景
- Recognizes the L1 or Calprotectin molecule, also called S100A8/9 and MRP8/14, an intra-cytoplasmic antigen comprising of a 12 kDa alpha chain and a 14 kDa beta chain. Calprotectin comprises 60 % of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Calprotectin has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. This mAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and is important for identifying macrophages in tissue sections.
- 途径
- S100 Proteins
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