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Nuclear Antigen 抗体

IPO-38 适用: 人, 小鼠, 大鼠 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal IPO-38 unconjugated
产品编号 ABIN3025764
发货至: 中国
  • 抗原 See all Nuclear Antigen (IPO-38) 抗体
    Nuclear Antigen (IPO-38)
    适用
    人, 小鼠, 大鼠
    宿主
    • 5
    小鼠
    克隆类型
    • 5
    单克隆
    标记
    • 5
    This Nuclear Antigen antibody is un-conjugated
    应用范围
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    PEG precipitation
    免疫原
    Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the IPO-38 antibody.
    克隆位点
    IPO-38
    亚型
    IgM kappa
    Top Product
    Discover our top product IPO-38 Primary Antibody
  • 应用备注
    Optimal dilution of the IPO-38 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    储存液
    Azide free
    储存条件
    4 °C,-20 °C
    储存方法
    Store the IPO-38 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    Nuclear Antigen (IPO-38)
    别名
    IPO-38 (IPO-38 产品)
    背景
    Recognizes a protein of 14-16  kDa, which is a novel nuclear antigen of proliferating cells. IPO38 antigen is present in the nuclei of proliferating cells such as Hodgkin s disease and non-Hodgkin s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO38 may be a useful marker of cell proliferation during monitoring of tumor progression.
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