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Nucleolin 抗体

NCL 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 豚鼠, 小鸡, Cat, Gerbil WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal NCL-902 unconjugated
产品编号 ABIN3025707
发货至: 中国
  • 抗原 See all Nucleolin (NCL) 抗体
    Nucleolin (NCL)
    适用
    • 128
    • 30
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    • 9
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    人, 小鼠, 大鼠, Cow, Pig, 兔, 豚鼠, 小鸡, Cat, Gerbil
    宿主
    • 88
    • 43
    • 1
    小鼠
    克隆类型
    • 88
    • 44
    单克隆
    标记
    • 63
    • 12
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    This Nucleolin antibody is un-conjugated
    应用范围
    • 103
    • 61
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    • 19
    • 16
    • 14
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    • 14
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    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Protein G affinity chromatography
    免疫原
    Recombinant human protein was used as the immunogen for the Nucleolin antibody.
    克隆位点
    NCL-902
    亚型
    IgG1
    Top Product
    Discover our top product NCL Primary Antibody
  • 应用备注
    Optimal dilution of the Nucleolin antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,IF: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Western blot: 0.5-1 μg/mL,Prediluted format: incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    0.2 mg/mL
    缓冲液
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    Nucleolin (NCL)
    别名
    Nucleolin (NCL 产品)
    别名
    C23 antibody, B530004O11Rik antibody, D0Nds28 antibody, D1Nds28 antibody, Nucl antibody, wu:fa12d03 antibody, zgc:152810 antibody, nucl antibody, LOC100282923 antibody, LOC100283058 antibody, nucleolin antibody, nucleolin S homeolog antibody, NCL antibody, Ncl antibody, ncl.S antibody, ncl antibody, LOC100195537 antibody, LOC100282923 antibody, LOC100283058 antibody
    背景
    Recognizes a protein of ~76  kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.
    途径
    Ribonucleoprotein Complex Subunit Organization
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