P27 抗体
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- 抗原
- P27
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适用
- 人, 小鼠, 大鼠, 猴
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (IF)
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Mouse recombinant protein was used as the immunogen for the p27 antibody.
- 克隆位点
- DCS-72-F6
- 亚型
- IgG1 kappa
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anti-P27 antibody
适用: 人, 小鼠, 大鼠, 猴 WB, IHC (p), FACS, IF 宿主: 小鼠 Monoclonal DCS-72-F6-KIP1-769 unconjugated
anti-P27 antibody适用: 人, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-P27 (Ser235), (Ser236) antibody适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-P27 (pThr198) antibody适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-P27 (pSer10) antibody适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-P27 (pThr187) antibody适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-P27 antibody适用: 人 WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-P27 (Ser235) antibody适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Optimal dilution of the p27 antibody to be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the p27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- P27
- 物质类
- Viral Protein
- 背景
- Recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.
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