HLA-DP/DR 抗体
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- 抗原 See all HLA-DP/DR products
- HLA-DP/DR (MHC Class II HLA-DP/DR (HLA-DP/DR))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HLA-DP/DR antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- 产品特性
- Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DR and DP. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36 kDa) and a chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Human REH cells were used as the immunogen for the HLA-DR antibody (HLA-DP).
- 克隆位点
- Bra-14
- 亚型
- IgG3 kappa
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anti-MHC Class II HLA-DP/DR (HLA-DP/DR) antibody
HLA-DP/DR 适用: 人 FACS, IP, WB, IHC (p) 宿主: 小鼠 Monoclonal MEM-136 unconjugated
anti-MHC Class II HLA-DP/DR (HLA-DP/DR) antibodyHLA-DP/DR 适用: 人, Pig FACS, IP, WB, IHC (p) 宿主: 小鼠 Monoclonal HL-38 unconjugated
anti-MHC Class II HLA-DP/DR (HLA-DP/DR) antibody (FITC)HLA-DP/DR 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-136 FITC
anti-MHC Class II HLA-DP/DR (HLA-DP/DR) antibody (Biotin)HLA-DP/DR 适用: 人 FACS, IP, WB, IHC (p) 宿主: 小鼠 Monoclonal MEM-136 Biotin
anti-MHC Class II HLA-DP/DR (HLA-DP/DR) antibody (PE)HLA-DP/DR 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-136 PE
anti-MHC Class II HLA-DP/DR (HLA-DP/DR) antibody (APC)HLA-DP/DR 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-136 APC
anti-MHC Class II HLA-DP/DR (HLA-DP/DR) antibody (FITC)HLA-DP/DR 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal HL-38 FITC
anti-MHC Class II HLA-DP/DR (HLA-DP/DR) antibody (PE)HLA-DP/DR 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal HL-38 PE
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- 应用备注
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Optimal dilution of the HLA-DR antibody (HLA-DP) should be determined by the researcher.
1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the HLA-DR antibody (HLA-DP) at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- HLA-DP/DR (MHC Class II HLA-DP/DR (HLA-DP/DR))
- 别名
- HLA-DR (HLA-DP) (HLA-DP/DR 产品)
- 背景
- Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DR and DP. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36 kDa) and a chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
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