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Neurofilament 抗体

适用: 人, 大鼠, 小鼠, Pig, 小鸡 FACS, IHC (p) 宿主: 小鼠 Monoclonal NF421 unconjugated
产品编号 ABIN3025341
发货至: 中国
  • 抗原 See all Neurofilament products
    Neurofilament
    适用
    • 26
    • 23
    • 20
    • 14
    • 14
    • 3
    人, 大鼠, 小鼠, Pig, 小鸡
    宿主
    • 21
    • 7
    • 1
    小鼠
    克隆类型
    • 21
    • 8
    单克隆
    标记
    • 15
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
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    This Neurofilament antibody is un-conjugated
    应用范围
    • 17
    • 14
    • 9
    • 7
    • 6
    • 5
    • 2
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Protein G purified Neurofilament antibody
    免疫原
    Human recombinant Neurofilament protein was used as the immunogen. (1)
    克隆位点
    NF421
    亚型
    IgG1
  • 应用备注
    The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.

    1. The NF421 antibody is specific to the heavy subunit.
    2. FFPE tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (2),Prediluted format : incubate for 30 min at RT (3)

    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    储存液
    Azide free
    储存条件
    4 °C,-20 °C
    储存方法
    Store the Neurofilament antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    Neurofilament
    Abstract
    Neurofilament 产品
    背景
    Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68  kDa (NF-L), 160  kDa (NF-M) and 200  kDa (NF-H). The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.
    基因ID
    4744
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