Neurofilament (Light Chain) 抗体
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- 抗原
- Neurofilament (Light Chain)
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适用
- 人, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Recombinant human neurofilament light chain protein was used as the immunogen for the Neurofilament antibody.
- 克隆位点
- MSI27-1
- 亚型
- IgG1
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anti-Neurofilament (Light Chain) antibody
适用: 人, 大鼠, 小鼠 IHC (p), IF, ICC, WB 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament (Light Chain) antibody适用: 人, 大鼠 IHC (p), IF 宿主: 小鼠 Monoclonal MSI27-1 unconjugated
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- 应用备注
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Optimal dilution of the Neurofilament antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Neurofilament antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Neurofilament (Light Chain)
- 背景
- Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. [RefSeq]
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