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Calponin 抗体

适用: 人, 大鼠 IHC (p), IF, FACS 宿主: 小鼠 Monoclonal CNN1-832-CALP unconjugated
产品编号 ABIN3025302
发货至: 中国
  • 抗原 See all Calponin products
    Calponin
    适用
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    人, 大鼠
    宿主
    • 24
    • 15
    小鼠
    克隆类型
    • 27
    • 12
    单克隆
    标记
    • 24
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    This Calponin antibody is un-conjugated
    应用范围
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    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)
    纯化方法
    Protein G affinity chromatography
    免疫原
    Recombinant full-length human protein (CNN1/832) and crude human uterus extract (CALP) were used as the immunogen for the Calponin antibody cocktail.
    克隆位点
    CNN1-832-CALP
    亚型
    IgG
  • 应用备注
    Optimal dilution of the Calponin antibody cocktail should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    储存液
    Azide free
    储存条件
    4 °C,-20 °C
    储存方法
    Store the Calponin antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    Calponin
    Abstract
    Calponin 产品
    别名
    CalPoNin antibody, cpn-1 antibody, cpn-2 antibody
    背景
    Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34  kDa form and a 29  kDa form. Expression of the 29  kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34  kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
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