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- 抗原 See all GFM1 抗体
- GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This GFM1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
- 无交叉反应
- Insect, Bacteria
- 产品特性
- MAb MTC719 recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC719 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondrial antibody.
- 克隆位点
- MTC719
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product GFM1 Primary Antibody
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anti-G-Elongation Factor, Mitochondrial 1 (GFM1) (AA 511-751) antibody
GFM1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-G-Elongation Factor, Mitochondrial 1 (GFM1) antibodyGFM1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-G-Elongation Factor, Mitochondrial 1 (GFM1) (AA 482-751) antibodyGFM1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-G-Elongation Factor, Mitochondrial 1 (GFM1) (AA 1-158) antibodyGFM1 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-G-Elongation Factor, Mitochondrial 1 (GFM1) (C-Term) antibodyGFM1 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Mitochondrial antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.25-0.5 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Mitochondrial antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))
- 别名
- Mitochondrial (GFM1 产品)
- 别名
- COXPD1 antibody, EFG antibody, EFG1 antibody, EFGM antibody, EGF1 antibody, GFM antibody, hEFG1 antibody, AW545374 antibody, D3Wsu133e antibody, Gfm antibody, EF-G antibody, Efg antibody, G elongation factor mitochondrial 1 antibody, G elongation factor, mitochondrial 1 antibody, GFM1 antibody, Gfm1 antibody
- 背景
- MAb MTC719 recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC719 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
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